--- title: I2SysBio identifies the most effective extraction method to obtain high quality DNA under non-ideal conditions description: Mária Džunková’s research group from the Institute of Integrative Systems Biology (I2SysBio), located in the Science Park of the University of Valencia, has developed methods for extracting DNA from animals collected in the field under non-ideal conditions. In particular, they obtained quality DNA from a nudibranched species-shell-free marine molluscs with soft bodies and bright colours-in six of the 24 combinations performed. The paper has been published in Ecology and Evolution image: https://news.pcuv.es/hubfs/i2sysbio%20adn%20alta%20calidad.jpg --- [Contact](https://www.pcuv.es/en/contacto) ![](https://news.pcuv.es/hubfs/icons/location.svg)[How to get to the Park](https://www.pcuv.es/en/contacto) [Intranet](https://portalcliente.pcuv.es/) - [Castellano](https://news.pcuv.es/) - [Valencià](https://news.pcuv.es/va/) - [English](https://news.pcuv.es/en/) ![](https://news.pcuv.es/hubfs/icons/search.svg) [![PCUV](https://news.pcuv.es/hubfs/logo_pcuv.svg)](https://www.pcuv.es/en/) - [Who we are](https://www.pcuv.es/en/conocenos) - [Science Park](https://www.pcuv.es/en/conocenos/parc-cientific) - [FPCUV](https://www.pcuv.es/en/conocenos/fpcuv) - [Transparency Portal](https://www.pcuv.es/en/conocenos/portal-de-transparencia-front) - [Services](https://www.pcuv.es/en/servicios) - [General Services](https://www.pcuv.es/en/servicios/servicios-generales) - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/infraestructura-y-espacios) - [Companies](https://www.pcuv.es/en/servicios/apoyo-a-empresas-y-startup) - [Networking](https://www.pcuv.es/en/servicios/networking) - [Preferential access](https://www.pcuv.es/en/servicios/acceso-preferente) - [Training](https://www.pcuv.es/en/servicios/formacion) - [Networks](https://www.pcuv.es/en/servicios/redes) - [Establishing in the Park](https://www.pcuv.es/en/instalarse-en-el-parc) - [Choose the Park](https://www.pcuv.es/en/instalarse-en-el-parc/elige-el-parc) - [The companies in the Park](https://www.pcuv.es/en/empresas) - [The companies in the Park](https://www.pcuv.es/en/empresas/empresas) - [Company directory and search engine](https://www.pcuv.es/en/empresas/empresas/listado-empresas) - [Biotechnology](https://www.pcuv.es/en/empresas/empresas/biotecnologia) - [Information technology (ICT)](https://www.pcuv.es/en/empresas/empresas/tic) - [Energy and the Environment](https://www.pcuv.es/en/empresas/empresas/energia-y-medio-ambiente) - [Advanced Services](https://www.pcuv.es/en/empresas/empresas/servicios-avanzados) - [Industry, Materials and Nanotechnology](https://www.pcuv.es/en/empresas/empresas/industrial-materiales-nanotecnologia) - [Research institutes](https://www.pcuv.es/en/institutos-de-investigacion) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion) - [Cavanilles Institute of Biodiversity and Evolutionary Biology (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icbibe) - [Institute of Agrochemistry and Food Technologies (CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/iata) - [Institute for Integrative Systems Biology (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/i2sysbio) - [Institute for Molecular Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmol) - [Institute of Materials Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmuv) - [Institute of Corpuscular Physics (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/ific) - [Institute of Robotics and Information and Communication Technologies (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/irtic) - [Astronomical Observatory of the University of Valencia](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/oauv) - [News](https://www.pcuv.es/en/actualidad) - [News](https://news.pcuv.es/en/) - [Diary](https://www.pcuv.es/en/actualidad/eventos) - [FPCUV Projects](https://www.pcuv.es/en/actualidad/Proyectos-FPCUV) - [Publications](https://www.pcuv.es/en/actualidad/publicaciones) - [Ebooks](https://www.pcuv.es/en/actualidad/ebooks) - [Podcast](https://www.pcuv.es/en/actualidad/podcast) - [Intranet](https://www.pcuv.es/en/intranet) - [Who we are](https://www.pcuv.es/en/conocenos) - [Science Park](https://www.pcuv.es/en/conocenos/parc-cientific) - [FPCUV](https://www.pcuv.es/en/conocenos/fpcuv) - [Transparency Portal](https://www.pcuv.es/en/conocenos/portal-de-transparencia-front) - [Services](https://www.pcuv.es/en/servicios) - [General Services](https://www.pcuv.es/en/servicios/servicios-generales) - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/infraestructura-y-espacios) - [Companies](https://www.pcuv.es/en/servicios/apoyo-a-empresas-y-startup) - [Networking](https://www.pcuv.es/en/servicios/networking) - [Preferential access](https://www.pcuv.es/en/servicios/acceso-preferente) - [Training](https://www.pcuv.es/en/servicios/formacion) - [Networks](https://www.pcuv.es/en/servicios/redes) - [Establishing in the Park](https://www.pcuv.es/en/instalarse-en-el-parc) - [Choose the Park](https://www.pcuv.es/en/instalarse-en-el-parc/elige-el-parc) - [Companies](https://www.pcuv.es/en/empresas) - [The companies in the Park](https://www.pcuv.es/en/empresas/empresas) - [Company directory and search engine](https://www.pcuv.es/en/empresas/empresas/listado-empresas) - [Biotechnology](https://www.pcuv.es/en/empresas/empresas/biotecnologia) - [Information technology (ICT)](https://www.pcuv.es/en/empresas/empresas/tic) - [Energy and the Environment](https://www.pcuv.es/en/empresas/empresas/energia-y-medio-ambiente) - [Advanced Services](https://www.pcuv.es/en/empresas/empresas/servicios-avanzados) - [Industry, Materials and Nanotechnology](https://www.pcuv.es/en/empresas/empresas/industrial-materiales-nanotecnologia) - [Research institutes](https://www.pcuv.es/en/institutos-de-investigacion) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion) - [Cavanilles Institute of Biodiversity and Evolutionary Biology (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icbibe) - [Institute of Agrochemistry and Food Technologies (CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/iata) - [Institute for Integrative Systems Biology (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/i2sysbio) - [Institute for Molecular Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmol) - [Institute of Materials Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmuv) - [Institute of Corpuscular Physics (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/ific) - [Institute of Robotics and Information and Communication Technologies (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/irtic) - [Astronomical Observatory of the University of Valencia](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/oauv) - [News](https://www.pcuv.es/en/actualidad) - [News](https://news.pcuv.es/en/) - [Diary](https://www.pcuv.es/en/actualidad/eventos) - [FPCUV Projects](https://www.pcuv.es/en/actualidad/Proyectos-FPCUV) - [Publications](https://www.pcuv.es/en/actualidad/publicaciones) - [Ebooks](https://www.pcuv.es/en/actualidad/ebooks) - [Podcast](https://www.pcuv.es/en/actualidad/podcast) - [Contact](https://www.pcuv.es/en/contacto) - [Intranet](https://portalcliente.pcuv.es/) 1. [Home](https://www.pcuv.es/en/home) 2. [News and Calendar](https://news.pcuv.es/) 3. News # I2SysBio identifies the most effective extraction method to obtain high quality DNA under non-ideal conditions 05/06/2025 Share this news: - ![](https://news.pcuv.es/hubfs/Blog/images/share.svg) - ![](https://news.pcuv.es/hubfs/Blog/images/printer.svg) ![](https://news.pcuv.es/hubfs/i2sysbio%20adn%20alta%20calidad.jpg) ## Mária Džunková’s research group from the Institute of Integrative Systems Biology (I2SysBio), located in the Science Park of the University of Valencia, has developed methods for extracting DNA from animals collected in the field under non-ideal conditions. In particular, they obtained quality DNA from a nudibranched species-shell-free marine molluscs with soft bodies and bright colours-in six of the 24 combinations performed. The paper has been published in *Ecology and Evolution* This research is part of the Catalan Initiative for a n Earth Biogenome Project (CBP), a regional branch of the Earth BioGenome Project (EBP), which aims to sequence and catalogue the genomes of all known eukaryotic species. Specifically, the CBP aims to sequence the genomes of more than 40,000 species that inhabit Catalan-speaking territories. In the published study, 24 combinations of conservation and DNA extraction were evaluated in one nudibranched species of *Peltodoris atromaculata*. Because nudibranchs are often collected in remote locations, conservation methods need to be applied which may affect the quality of the DNA, thus making it difficult to obtain the material required by long-read sequencing techniques, those that allow very precise and continuous reading assemblies to be generated, which are essential for the study of genomic architecture and the resolution of complex regions of the genome. > "The results indicate that the success of high molecular weight DNA extractions is influenced by conservation methods. The study provides key results by identifying effective combinations of DNA conservation and extraction, which will generate high-quality reference genomes and contribute to the knowledge and sustainable use of marine biodiversity", Mária Džunková, I2SysBio researcher "The results indicate that the success of high molecular weight DNA extractions is influenced by conservation methods," explains Mária Džunková, who notes that the study provides key findings in identifying effective combinations of DNA conservation and extraction, "which will enable the generation of high quality reference genomes and contribute to the knowledge and sustainable use of marine biodiversity". Although only 1% of the 2,545 known nudibranch species have now been sequenced, there is growing interest in this group for its outstanding biological diversity and complex chemical defense mechanisms; with potential in biotechnology and pharmacology. In this research have also participated Inés Alberola-Mora, Oleanna Guerra-Font and Omar Daniel Espinoza-Calderón, research staff of the [Institute of Integrative Systems Biology (I2SysBio)](https://www.pcuv.es/es/institutos-de-investigacion/i2sysbio), located in the scientific areaacademic of the [Science Park of the University of Valencia (PCUV)](https://www.pcuv.es/es/), as well as joint centre of the Superior Council for Scientific Research (CSIC) and the University of Valencia (UV). Carles Galià-Camps from the University of Barcelona, the Biodiversity Research Institute (IRBo) and the Blanes Centre for Advanced Studies (CSIC) have also participated. Research has been supported by the Institut d'Estudis Catalans, the Generalitat Valenciana and the Ministry of Science, Innovation and Universities. *Fuente: UV Noticias* *Don’t miss the interview with Maria Dzunkova, researcher of I2SysBio, in our section of Europark*     Reference -- Inés Alberola-Mora, Oleanna Guerra-Font, Omar Daniel Espinoza-Calderón, Carles Galià-Camps, Mária Džunková: «Combination of Sample Preservation Approaches and DNA Extraction Methods for Long-Read Sequencing of Nudibranchs’ Genomes». *Ecology and Evolution*, 2025; 15:e71262. DOI: [https://doi.org/10.1002/ece3.71262](https://doi.org/10.1002/ece3.71262) --   ### Recent Posts     [![PCUV](https://news.pcuv.es/hubfs/icons/logo.svg)](https://www.pcuv.es/en/home) ## Subscribe to our **newsletter** - Get to know us - [Parc Científic](https://www.pcuv.es/en/conocenos) - [FPCUV](https://www.pcuv.es/en/fpcuv/) - [Transparency Portal](https://www.pcuv.es/en/portal-de-transparencia) - --- Services - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/espacios-para-empresas) - [Support for companies and startups](https://www.pcuv.es/en/servicios/apoyo-a-emprededor-empresas) - [Networking](https://www.pcuv.es/en/servicios/networking-empresas/) - [UV services](https://www.pcuv.es/en/servicios/servicios-uv) - [Training](https://www.pcuv.es/en/servicios/formacion/) - [Networks](https://www.pcuv.es/en/servicios/redes/) - Establishing in the park - [Establishing in the park](https://www.pcuv.es/en/instalarse-en-el-parc/) - --- Companies - [The park's companies](https://www.pcuv.es/en/empresas) - [Company directory and search engine](https://www.pcuv.es/en/directorio-buscador-empresas) - Sectors - [Biotechnology, agrifood, medicine and health](https://www.pcuv.es/en/empresas-biotecnologia-medicina-salud) - [Information and communication technologies ICT](https://www.pcuv.es/en/empresas-tecnologias-informacion-comunicacion-tic) - [Energy and environment](https://www.pcuv.es/en/empresas-energia-y-medio-ambiente) - [Advanced services](https://www.pcuv.es/en/empresas-servicios-avanzados) - [Industry, materials and nanotechnology](https://www.pcuv.es/en/empresas-industrial-materiales-nanotecnologia) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion) - PCUV News - [News](https://news.pcuv.es/en/) - [Calendar](https://www.pcuv.es/en/agenda-eventos/) - [Park projects](https://www.pcuv.es/en/proyectos-del-parc/) - [Publications](https://www.pcuv.es/en/publicaciones/) - [Ebooks](https://www.pcuv.es/en/ebooks/) - [Podcasts](https://www.pcuv.es/en/podcasts/) - ![](https://news.pcuv.es/hubfs/icons/location-f.svg)[How to get to the park](https://www.pcuv.es/en/contacto#map) - --- [Contact](https://www.pcuv.es/en/contacto) - --- [Internal information channel](https://pcuv.es/en/canal-interno-de-informacion) - - - - - - --- ©2026 Parc Científic de la Universitat de València - [Cookies policy](https://www.pcuv.es/en/politica-cookies) - [Privacy policy](https://www.pcuv.es/en/politica-privacidad) - [Legal notice](https://www.pcuv.es/en/aviso-legal-web)