--- title: I2SysBio scientists explore a new gene sequencing technique that helps decipher diseases like cancer description: Researchers at the Institute of Integrative Systems Biology (I2SysBio) have published a review of long-read transcriptomic sequencing technology, which allows RNA molecules present in all cells and containing genetic information essential for life to be analysed more precisely than traditional methods. This technique is essential for a better understanding of ageing, neurodegenerative diseases or cancer. Article published in Nature Reviews Genetics image: https://news.pcuv.es/hubfs/noti%20i2sysbio%20secuenciaci%C3%B3n%20gen%C3%A9tica.jpg --- [Contact](https://www.pcuv.es/en/contacto) ![](https://news.pcuv.es/hubfs/icons/location.svg)[How to get to the Park](https://www.pcuv.es/en/contacto) [Intranet](https://portalcliente.pcuv.es/) - [Castellano](https://news.pcuv.es/) - [Valencià](https://news.pcuv.es/va/) - [English](https://news.pcuv.es/en/) ![](https://news.pcuv.es/hubfs/icons/search.svg) [![PCUV](https://news.pcuv.es/hubfs/logo_pcuv.svg)](https://www.pcuv.es/en/) - [Who we are](https://www.pcuv.es/en/conocenos) - [Science Park](https://www.pcuv.es/en/conocenos/parc-cientific) - [FPCUV](https://www.pcuv.es/en/conocenos/fpcuv) - [Transparency Portal](https://www.pcuv.es/en/conocenos/portal-de-transparencia-front) - [Services](https://www.pcuv.es/en/servicios) - [General Services](https://www.pcuv.es/en/servicios/servicios-generales) - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/infraestructura-y-espacios) - [Companies](https://www.pcuv.es/en/servicios/apoyo-a-empresas-y-startup) - [Networking](https://www.pcuv.es/en/servicios/networking) - [Preferential access](https://www.pcuv.es/en/servicios/acceso-preferente) - [Training](https://www.pcuv.es/en/servicios/formacion) - [Networks](https://www.pcuv.es/en/servicios/redes) - [Establishing in the Park](https://www.pcuv.es/en/instalarse-en-el-parc) - [Choose the Park](https://www.pcuv.es/en/instalarse-en-el-parc/elige-el-parc) - [The companies in the Park](https://www.pcuv.es/en/empresas) - [The companies in the Park](https://www.pcuv.es/en/empresas/empresas) - [Company directory and search engine](https://www.pcuv.es/en/empresas/empresas/listado-empresas) - [Biotechnology](https://www.pcuv.es/en/empresas/empresas/biotecnologia) - [Information technology (ICT)](https://www.pcuv.es/en/empresas/empresas/tic) - [Energy and the Environment](https://www.pcuv.es/en/empresas/empresas/energia-y-medio-ambiente) - [Advanced Services](https://www.pcuv.es/en/empresas/empresas/servicios-avanzados) - [Industry, Materials and Nanotechnology](https://www.pcuv.es/en/empresas/empresas/industrial-materiales-nanotecnologia) - [Research institutes](https://www.pcuv.es/en/institutos-de-investigacion) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion) - [Cavanilles Institute of Biodiversity and Evolutionary Biology (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icbibe) - [Institute of Agrochemistry and Food Technologies (CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/iata) - [Institute for Integrative Systems Biology (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/i2sysbio) - [Institute for Molecular Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmol) - [Institute of Materials Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmuv) - [Institute of Corpuscular Physics (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/ific) - [Institute of Robotics and Information and Communication Technologies (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/irtic) - [Astronomical Observatory of the University of Valencia](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/oauv) - [News](https://www.pcuv.es/en/actualidad) - [News](https://news.pcuv.es/en/) - [Diary](https://www.pcuv.es/en/actualidad/eventos) - [FPCUV Projects](https://www.pcuv.es/en/actualidad/Proyectos-FPCUV) - [Publications](https://www.pcuv.es/en/actualidad/publicaciones) - [Ebooks](https://www.pcuv.es/en/actualidad/ebooks) - [Podcast](https://www.pcuv.es/en/actualidad/podcast) - [Intranet](https://www.pcuv.es/en/intranet) - [Who we are](https://www.pcuv.es/en/conocenos) - [Science Park](https://www.pcuv.es/en/conocenos/parc-cientific) - [FPCUV](https://www.pcuv.es/en/conocenos/fpcuv) - [Transparency Portal](https://www.pcuv.es/en/conocenos/portal-de-transparencia-front) - [Services](https://www.pcuv.es/en/servicios) - [General Services](https://www.pcuv.es/en/servicios/servicios-generales) - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/infraestructura-y-espacios) - [Companies](https://www.pcuv.es/en/servicios/apoyo-a-empresas-y-startup) - [Networking](https://www.pcuv.es/en/servicios/networking) - [Preferential access](https://www.pcuv.es/en/servicios/acceso-preferente) - [Training](https://www.pcuv.es/en/servicios/formacion) - [Networks](https://www.pcuv.es/en/servicios/redes) - [Establishing in the Park](https://www.pcuv.es/en/instalarse-en-el-parc) - [Choose the Park](https://www.pcuv.es/en/instalarse-en-el-parc/elige-el-parc) - [Companies](https://www.pcuv.es/en/empresas) - [The companies in the Park](https://www.pcuv.es/en/empresas/empresas) - [Company directory and search engine](https://www.pcuv.es/en/empresas/empresas/listado-empresas) - [Biotechnology](https://www.pcuv.es/en/empresas/empresas/biotecnologia) - [Information technology (ICT)](https://www.pcuv.es/en/empresas/empresas/tic) - [Energy and the Environment](https://www.pcuv.es/en/empresas/empresas/energia-y-medio-ambiente) - [Advanced Services](https://www.pcuv.es/en/empresas/empresas/servicios-avanzados) - [Industry, Materials and Nanotechnology](https://www.pcuv.es/en/empresas/empresas/industrial-materiales-nanotecnologia) - [Research institutes](https://www.pcuv.es/en/institutos-de-investigacion) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion) - [Cavanilles Institute of Biodiversity and Evolutionary Biology (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icbibe) - [Institute of Agrochemistry and Food Technologies (CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/iata) - [Institute for Integrative Systems Biology (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/i2sysbio) - [Institute for Molecular Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmol) - [Institute of Materials Science (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/icmuv) - [Institute of Corpuscular Physics (UV + CSIC)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/ific) - [Institute of Robotics and Information and Communication Technologies (UV)](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/irtic) - [Astronomical Observatory of the University of Valencia](https://www.pcuv.es/en/institutos-de-investigacion/institutos-de-investigacion/oauv) - [News](https://www.pcuv.es/en/actualidad) - [News](https://news.pcuv.es/en/) - [Diary](https://www.pcuv.es/en/actualidad/eventos) - [FPCUV Projects](https://www.pcuv.es/en/actualidad/Proyectos-FPCUV) - [Publications](https://www.pcuv.es/en/actualidad/publicaciones) - [Ebooks](https://www.pcuv.es/en/actualidad/ebooks) - [Podcast](https://www.pcuv.es/en/actualidad/podcast) - [Contact](https://www.pcuv.es/en/contacto) - [Intranet](https://portalcliente.pcuv.es/) 1. [Home](https://www.pcuv.es/en/home) 2. [News and Calendar](https://news.pcuv.es/) 3. News # I2SysBio scientists explore a new gene sequencing technique that helps decipher diseases like cancer 03/06/2025 Share this news: - ![](https://news.pcuv.es/hubfs/Blog/images/share.svg) - ![](https://news.pcuv.es/hubfs/Blog/images/printer.svg) ![](https://news.pcuv.es/hubfs/noti%20i2sysbio%20secuenciaci%C3%B3n%20gen%C3%A9tica.jpg) ## Researchers at the Institute of Integrative Systems Biology (I2SysBio) have published a review of long-read transcriptomic sequencing technology, which allows RNA molecules present in all cells and containing genetic information essential for life to be analysed more precisely than traditional methods. This technique is essential for a better understanding of ageing, neurodegenerative diseases or cancer. Article published in *Nature Reviews Genetics* Transcriptomics is the study of all RNA (ribonucleic acid) molecules present in a cell or tissue. These are intermediate molecules between DNA and proteins. While DNA contains the genetic information, RNA is the one that allows protein synthesis and the information to be understood by cells. It allows us to understand which genes are active at a given time and what their degree of activation is in different organisms, which is key to understanding the origin and development of many human diseases. It can also be used to study drought or heat resistant plants, to examine less studied organisms such as viruses and bacteria, and was even used by CSIC scientific staff who recently visited Antarctica to study avian influenza. As explained by Ana Conesa, research professor at the [Institute of Integrative Systems Biology (I2SysBio)](https://www.pcuv.es/es/institutos-de-investigacion/i2sysbio), located in the scientific-academic area of the [Science Park of the University of Valencia (PCUV)](https://www.pcuv.es/es/), in addition to joint center of the Superior Council for Scientific Research (CSIC) and the University of Valencia (UV), ensures that "With this research we have provided the scientific community with an essential guide to understanding long-read transcriptomic sequencing technology, both experimental workflows and computational analyses. We present a comprehensive overview of long-read RNA sequencing techniques and cover from experimental design to data processing, identification of different RNA molecules and subsequent analysis". Until a few years ago, transcriptomics were studied using short-reading sequencing, which involved breaking RNA molecules into small fragments of between 50 and 300 nucleotides, basic units of the DNA and RNA nucleic acids (the famous letters ATCG). This meant that in order to find the original RNA molecule, the puzzle of RNA fragments had to be reconstructed, which caused a great number of errors, as there were very similar sequences between RNAs. > "These studies are of interest because they could contribute to progress in the understanding of neuropsychiatric and neurodegenerative diseases. Currently, it is known that there are differences in the isoform expressed in different regions of the brain and that they are associated with multiple neuronal complications", Carolina Monzó, I2SysBio researcher As I2SysBio’s fellow researcher Carolina Monzó explains, "long-reading sequencing reads fragments of thousands of nucleotides, allowing complete molecules to be sequenced and RNA to be studied without the need for puzzles. In addition, by sequencing RNA molecules one by one, long readings make it possible to identify their small modifications, called epitranscriptomics, which are fundamental for their function. This was not possible with previous technologies". ## Scientific applications By obtaining sequences of complete RNA molecules, this technology facilitates all kinds of applications and studies where it is important to know precisely the composition and structure of RNA. For example: expression of isoforms (different forms of RNA from the same gene), discovery of new forms of RNA, the annotation of genes in genomes of poorly studied species or the study of differential RNA expression between that produced by the chromosome inherited from the father and the chromosome inherited from the mother, among others. According to Monzó, "these studies are interesting because they could contribute to the progress in understanding neuropsychiatric and neurodegenerative diseases. At present, it is known that there are differences in the isoform expressed in different regions of the brain and that they are associated with multiple neuronal complications". Tianyuan Liu, a pre-doctoral student from the Genomic Group of Gene Expression at I2SysBio, also participated in the article. Currently, he has a pre-doctoral contract under the Marie Skłodowska-Curie Actions Doctoral Network LongTREC of the European Union. *Source: UV News*   Reference -- Monzó, C., Liu, T. & Conesa, A.  Transcriptomics in the era of long-read sequencing. *Nat Rev Genet* (2025). DOI: [https://doi.org/10.1038/s41576-025-00828-z](https://doi.org/10.1038/s41576-025-00828-z) --   ### Recent Posts     [![PCUV](https://news.pcuv.es/hubfs/icons/logo.svg)](https://www.pcuv.es/en/home) ## Subscribe to our **newsletter** - Get to know us - [Parc Científic](https://www.pcuv.es/en/conocenos) - [FPCUV](https://www.pcuv.es/en/fpcuv/) - [Transparency Portal](https://www.pcuv.es/en/portal-de-transparencia) - --- Services - [Infrastructures and spaces](https://www.pcuv.es/en/servicios/espacios-para-empresas) - [Support for companies and startups](https://www.pcuv.es/en/servicios/apoyo-a-emprededor-empresas) - [Networking](https://www.pcuv.es/en/servicios/networking-empresas/) - [UV services](https://www.pcuv.es/en/servicios/servicios-uv) - [Training](https://www.pcuv.es/en/servicios/formacion/) - [Networks](https://www.pcuv.es/en/servicios/redes/) - Establishing in the park - [Establishing in the park](https://www.pcuv.es/en/instalarse-en-el-parc/) - --- Companies - [The park's companies](https://www.pcuv.es/en/empresas) - [Company directory and search engine](https://www.pcuv.es/en/directorio-buscador-empresas) - Sectors - [Biotechnology, agrifood, medicine and health](https://www.pcuv.es/en/empresas-biotecnologia-medicina-salud) - [Information and communication technologies ICT](https://www.pcuv.es/en/empresas-tecnologias-informacion-comunicacion-tic) - [Energy and environment](https://www.pcuv.es/en/empresas-energia-y-medio-ambiente) - [Advanced services](https://www.pcuv.es/en/empresas-servicios-avanzados) - [Industry, materials and nanotechnology](https://www.pcuv.es/en/empresas-industrial-materiales-nanotecnologia) - [Research Institutes](https://www.pcuv.es/en/institutos-de-investigacion) - PCUV News - [News](https://news.pcuv.es/en/) - [Calendar](https://www.pcuv.es/en/agenda-eventos/) - [Park projects](https://www.pcuv.es/en/proyectos-del-parc/) - [Publications](https://www.pcuv.es/en/publicaciones/) - [Ebooks](https://www.pcuv.es/en/ebooks/) - [Podcasts](https://www.pcuv.es/en/podcasts/) - ![](https://news.pcuv.es/hubfs/icons/location-f.svg)[How to get to the park](https://www.pcuv.es/en/contacto#map) - --- [Contact](https://www.pcuv.es/en/contacto) - --- [Internal information channel](https://pcuv.es/en/canal-interno-de-informacion) - - - - - - --- ©2026 Parc Científic de la Universitat de València - [Cookies policy](https://www.pcuv.es/en/politica-cookies) - [Privacy policy](https://www.pcuv.es/en/politica-privacidad) - [Legal notice](https://www.pcuv.es/en/aviso-legal-web)